AAV expression is a process by which a virus or plasmid is expressed in cells. It can be used to introduce AAV genes into cells for therapeutic purposes, or to study the effects of AAV on cells.
There are several methods for expressing AAV genes in cells. The most common method is called transient transfection, where the virus or plasmid is inserted into the cells, and then the cells are allowed to grow and divide. Navigate to this site for more information about aav expression system.
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Once the cells have reached an appropriate size, the viral genome is transfected into them using electroporation. This method is used to transfer large pieces of genetic material into cells, and it is often used to introduce genes that encode proteins that are too large to be inserted using other methods.
Another method called stable transfection uses a vector that contains a copy of the virus or plasmid DNA. The vector is inserted into the cell using standard techniques, and the gene encoding the AAV protein is then copied into the vector. The vector can then be left in the cell, or it can be removed and discarded after it has done its job.
This method is used when it is necessary to keep track of which cells have been transfected with the virus.When it is desired to remove the vector from the cells, a drug called a transfection inhibitor is used.